Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used for immunostaining

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques:

Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used for fluorescence activated cell sorting

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques: Fluorescence

Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used in Western Blot analysis

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques: Western Blot

Journal: Discover oncology

Article Title: An integrative analysis combining bioinformatics, network pharmacology and experimental methods identified key genes of EGCG targets in Nasopharyngeal Carcinoma.

doi: 10.1007/s12672-025-02365-x

Figure Lengend Snippet: Fig. 7 To identify potential therapeutic targets for EGCG. A Expression of CYCS in the GEO cohort. B Visualized the distribution of the six- genes in the risk model within the previous volcano plot. C Expression of MYL12B in the GEO cohort

Article Snippet: Following this step, primary antibodies against CYCS (diluted 1:1000, #10,993–1-AP, Proteintech), and MYL12B (diluted 1:250, #10,324–1-AP, Proteintech) were applied, and the sections were left to incubate with the secondary antibody (kit-5020, MAIXIN BIOTECH, Fuzhou, China) for 70 min at room temperature.

Techniques: Biomarker Discovery, Expressing

Journal: Discover oncology

Article Title: An integrative analysis combining bioinformatics, network pharmacology and experimental methods identified key genes of EGCG targets in Nasopharyngeal Carcinoma.

doi: 10.1007/s12672-025-02365-x

Figure Lengend Snippet: Fig. 8 Immunohistochemistry of CYCS, and MYL12B. A, B CYCS protein levels in NPC tissue expression. C Diagnostic efficacy of CYCS in NPC based on protein level. D, E MYL12B protein levels in NPC tissue expression. F Diagnostic efficacy of MYL12B in NPC based on protein level

Article Snippet: Following this step, primary antibodies against CYCS (diluted 1:1000, #10,993–1-AP, Proteintech), and MYL12B (diluted 1:250, #10,324–1-AP, Proteintech) were applied, and the sections were left to incubate with the secondary antibody (kit-5020, MAIXIN BIOTECH, Fuzhou, China) for 70 min at room temperature.

Techniques: Immunohistochemistry, Expressing, Diagnostic Assay

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 4. Effect of yak milk on immunohistochemistry of kidney in mice. (A) Images of mouse kidney tissue cross sections immunostained for TRPV5 and calbindin-D28k (scale bar = 100 μm). (B) Relative mean density of TRPV5 in mouse kidney. (C) Relative mean density of calbindin-D28k in mouse kidney. Mean density = (integrated optical density [IOD] sum)/area. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Immunohistochemistry

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 5. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k in mouse kidney. (A) Western blot showing the expression of TRPV5 and calbindin-D28k in mouse kidney. (B) Quantification of TRPV5 expression from the Western blots. (C) Quantification of calbindin-D28k expression from the Western blots. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with dif- ferent lowercase letters (a–c) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing, Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 6. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k mRNA in mouse kidney. (A) Relative expression of TRPV5 mRNA in mouse kidney. (B) Relative expression of calbindin-D28k mRNA in mouse kidney. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing